期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1515, 期 -, 页码 146-153出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.07.089
关键词
Protein chromatography modeling; Root cause investigation; Artificial neural networks; Ion-exchange chromatography
资金
- European Union's Horizon Research and Innovation Programme [635557]
In protein chromatography, process variations, such as aging of column or process errors, can result in deviations of the product and impurity levels. Consequently, the process performance described by purity, yield, or production rate may decrease. Based on visual inspection of the UV signal, it is hard to identify the source of the error and almost unfeasible to determine the quantity of deviation. The problem becomes even more pronounced, if multiple root causes of the deviation are interconnected and lead to an observable deviation. In the presented work, a novel method based on the combination of mechanistic chromatography models and the artificial neural networks is suggested to solve this problem. In a case study using a model protein mixture, the determination of deviations in column capacity and elution gradient length was shown. Maximal errors of 1.5% and 4.90% for the prediction of deviation in column capacity and elution gradient length respectively demonstrated the capability of this method for root cause investigation. (C) 2017 The Author(s). Published by Elsevier B.V.
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