Effects of ammonia on propionate degradation and microbial community in digesters using propionate as a sole carbon source

BACKGROUNDPropionate accumulation may lead to digester failure. This study aimed to investigate the effect of ammonia, a metabolic product of protein, on propionate degradation. The shift of microbial community was also investigated. RESULTSPropionate accumulated over the experimental period in the reactor with a total ammonia nitrogen (TAN) concentration of 2.5 g N L-1, as a result the digester failed after 4 hydraulic retention times (HRT) at an organic load rate (OLR) of 0.8 g propionic acid (HPr) L-1 d(-1). The average HPr degradation rate was below 54% during the fourth HRT, while >97% of the degraded HPr was converted to methane. The reactor without ammonia stress did not experience HPr accumulation and OLR was increased stepwise to 1.2 g L-1 d(-1) at the 8th HRT. The average HPr degradation rate and methane recovery rate of this reactor in the last HRT was 99% and 74%, respectively. According to the shifts of microbial community, acetoclastic methanogen was more vulnerable to ammonia than hydrogenotrophic methanogen CONCLUSIONTAN concentration of 2.5 g N L-1 inhibited propionate degradation more severely than methanogenesis. The loss of abundance of Clostridiaceae and Syntrophobacter might be the main reason for the poor performance under ammonia stress. (c) 2017 Society of Chemical Industry