期刊
RSC ADVANCES
卷 10, 期 11, 页码 6647-6653出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra00022a
关键词
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资金
- National Key Research and Development Program of China [2018YFC1602800]
- Key Scientific and Technological Project of Henan Province [192102310255]
- Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology [2016RCJH04]
An electrochemical aptasensor is fabricated for lead ion (Pb2+) detection based on catalytic hairpin assembly as signal amplification. Biomass porous carbon derived from soybean straw (PCs) is used to load platinum nanoparticles (PtNPs), which are introduced as mimetic enzymes to catalyze the hydroquinone-H2O2 system. In the presence of Pb2+, the complementary DNA (cDNA) releases from aptamer-cDNA and hybridizes with hairpin DNA1 (HP1) on the electrode surface. After bio-hairpin DNA2 (bio-HP2) is added, hybridization between HP1 and bio-HP2 further releases cDNA, which participates in the next cycle and triggers amplification, eventually forming a large number of bio-HP2/HP1 on the electrode surface. Then PtNPs@PCs immobilize on the electrode surface by specific binding of streptavidin with biotin, and catalyze the oxidation of hydroquinone in the presence of H2O2. The produced electrochemical signal depends on the concentration of Pb2+. The developed biosensor exhibits a wide linear range from 50 pM to 1000 nM with a detection limit of 18 pM, and high selectivity for Pb2+ over other environmentally relevant metal ions at concentration ratios of 100. The recoveries of Pb2+ in real samples were 93.5-108%. Thus, the proposed biosensor can provide an efficient method for sensitive and selective detection of Pb2+ in real samples.
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