期刊
JOURNAL OF CEREAL SCIENCE
卷 76, 期 -, 页码 186-192出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2017.04.011
关键词
Red rice polyphenols; Pancreatic alpha-Amylase; Inhibit; Molecular docking
资金
- China National Key Technology Research and Development Program [2012BAD37B08-3]
- National High Technology Research and Development Program 863 [2013AA102203-7]
- National Natural Science Foundation of China [31471617]
This study sought to investigate the possible inhibition mechanism of red rice polyphenols (RRP) on pancreatic alpha-amylase (PA) activity. RRP showed strong inhibition against PA activity and the half inhibitory concentration (IC50) value was 3.61 mu g/mL. The fluorescence quenching of PA by RRP was a combination of static quenching and dynamic quenching. RRP could aggregate with PA and the physiochemical properties of the aggregates were closely related to the concentration of RRP. Kinetic analysis suggested that the inhibition mode of RRP on PA was reversible inhibition, which was a mixing of competitive inhibition and noncompetitive inhibition. Molecular docking speculated that RRP could form hydrogen bonds with PA by binding to the catalytic active sites (ASP197, GLU233 and ASP300) and the microenvironments of TRP58 and TRP59 were altered, thus inhibiting PA activity. (C) 2017 Elsevier Ltd. All rights reserved.
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