Association of IL-10 (rs1800872) and IL-4R (rs1805010 ) polymorphisms with cervical intraepithelial lesions and cervical carcinomas

Purpose: Genetic characteristic of cytokines may influence cervical intraepithelial neoplasia (CIN) and cervical cancer (CCa) susceptibility. We analysed an association of IL-10-592 A/C, IL-4R I75VA/G polymorphisms with susceptibility to human papillomavirus (HPV) positive CIN and CCa. Methods: Using multiplex PCR- SNaPShot analysis, 134 cases (HPV positive CINs and CCa) and 113 controls (HPV negative NILM) were genotyped for these two cytokine variants. Results: Data analyzed using odds ratio (OR) and chi-square (x(2)) test showed that the frequency of CC of IL-10-592 genotype was significantly higher in cases (67.2%) than in controls (49.6%) [CC vs CA+AA; p =0.005, OR=2.08 (95%CI: 1.24-3.49)] as well as the allelic frequency of major C allele (82. 1%) in cases than in controls (72.6%) [p=0.01, OR=1.73 (95%CI: 1.13-2.66)]. Furthermore, AA genotype of IL-4R175V had significantly lower frequency in CINI (25.0%) compared with CIN2+ group (30.8%) (p=0.03, OR=0.39, 95%CI: 0.14-1.11) after the stratifications of the cases in low grade and high grade with CCa as separate groups. Conclusion: We concluded that IL-10-592 A/AA variant indicates a protective role in cervical cancer development and the GG genotype of IL-4RI75V conferred protection against progression of CIN1 to CIN2+ or CCa among women from Republic of North Macedonia.