期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 233, 期 5, 页码 3968-3981出版社
WILEY
DOI: 10.1002/jcp.26176
关键词
cancer; DNA methylation; epigenetic; genome-wide analysis
DNA methylation was the first epigenetic modification to be detected in human cancers with specific relation to aberrant gene expression. Herein, DNA methylation analysis explains how epigenetic patterns affect gene expression level. Hypermethylation at tumor suppressor gene loci leads to increased tumorigenesis due to tumor suppressor gene silencing, whereas global hypomethylation of CpG islands (CGIs) is followed by genomic instability and aberrant activation of multiple oncogenes. Therefore, characterization of the genes which silenced or activated epigenetically in human tumor cells can improve our understanding of cancer biology. Different genome-wide methodologies are applied to evaluate methylation status. Various commonly conducted techniques for this evaluation are reviewed in this paper. We provided comparative description of the procedures, advantages, and drawbacks of genome-wide DNA methylation analysis methods and biological applications, to give information on selecting the appropriate method for different methylation studies. Various commonly conducted techniques for this evaluation are reviewed in this paper. We provided a comparative description of the procedures, advantages, and drawbacks of genome-wide DNA methylation analysis methods and biological applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据