期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 118, 期 8, 页码 2064-2071出版社
WILEY
DOI: 10.1002/jcb.25811
关键词
ADRENOCORTICAL TUMORS; ADRENAL GLAND; TERT PROMOTER MUTATIONS; TELOMERASE EXPRESSION; N-CADHERIN EXPRESSION
资金
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/89308/2012]
- Fundo Europeu de Desenvolvimento Regional and National Funds [PEst-C/SAU/LA0003/2013]
- Portuguese Foundation for Science and Technology [UID/Multi/00215/2013]
- Programa Operacional Regional do Norte (ON.2 - O Novo Norte)
- Fundo Europeu de Desenvolvimento Regional (FEDER)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/89308/2012] Funding Source: FCT
Adrenocortical carcinomas (ACC) are most frequently highly aggressive tumors. We assessed the telomerase reverse transcriptase (TERT) and N-cadherin role in the biology of ACC and their potential utility as molecular biomarkers, in different types of tumoral adrenocortical tissue. A total of 48 adrenal cortex samples (39 tumoral and 9 normal adrenal glands) were studied. TERT promoter mutations were searched by PCR and Sanger sequencing in two hotspots positions (-124 and -146). Also, telomerase and N-cadherin expression were evaluated by immunohistochemistry. TERT promoter mutations were not detected in any of the samples either malignant or benign. Telomerase nuclear expression was present in 26.6% of ACC and in 45.5% of non-functioning adenomas. It was absent in benign Cushing's lesions and in normal adrenal glands. Contrarily, N-cadherin was always expressed in the cellular membranes of benign adenomas or normal adrenals but no expression was detected in the majority of ACC. Nuclear telomerase and membrane N-cadherin expression were positively correlated in ACCs. We conclude that in ACC, the loss of N-cadherin is a frequent phenomenon while the existence of TERT promoter mutations is not and nuclear telomerase expression is present in only a minority of cases. Since the loss of N-cadherin expression was identified in both high and low proliferative ACC, this marker should be considered important for diagnostic application. Our study also suggests the existence of a TERT non-canonical function in cell adhesion. J. Cell. Biochem. 118: 2064-2071, 2017. (c) 2017 Wiley Periodicals, Inc.
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