期刊
JOURNAL OF CELL SCIENCE
卷 130, 期 21, 页码 3698-3712出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.206201
关键词
Sensory cilia; Usher syndrome; Pcdh15; Integrin alpha 8
类别
资金
- National Institutes of Health [5P20RR018788]
- Tobacco Settlement Fund from the State of Nebraska
The way an organism perceives its surroundings depends on sensory systems and the highly specialized cilia present in the neurosensory cells. Here, we describe the existence of an integrin alpha 8 (Itga8) and protocadherin-15a (Pcdh15a) ciliary complex in neuromast hair cells in a zebrafish model. Depletion of the complex via downregulation or loss-of-function mutation leads to a dysregulation of cilia biogenesis and endocytosis. At the molecular level, removal of the complex blocks the access of Rab8a into the cilia as well as normal recruitment of ciliary cargo by centriolar satellites. These defects can be reversed by the introduction of a constitutively active form of Rhoa, suggesting that Itga8-Pcdh15a complex mediates its effect through the activation of this small GTPase and probably by the regulation of actin cytoskeleton dynamics. Our data points to a novel mechanism involved in the regulation of sensory cilia development, with the corresponding implications for normal sensory function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据