期刊
JOURNAL OF CELL SCIENCE
卷 130, 期 11, 页码 1904-1916出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.201178
关键词
3D tracking; Motor protein; Super-resolution microscopy; Vesicle trafficking
类别
资金
- Fundacion Cellex Barcelona, the European Research Council [337191-MOTORS]
- Ministerio de Economia y Competitividad
- Fonds europeen de developpement regional [FIS2015-63550-R]
- Severo Ochoa Programme for Centres of Excellence in RD [SEV-2015-0522]
- Deutsche Forschungsgemeinschaft [SFB1032 (Project B3)]
- Ludwig-Maximilians- Universitat Munchen via the LMUinnovativ program BioImaging Network
- Center for NanoScience, Ludwig-Maximilians-Universitat Munchen (CeNS)
Vesicle transport is regulated at multiple levels, including regulation by scaffolding proteins and the cytoskeleton. This tight regulation is essential, since slowing or stoppage of transport can cause accumulation of obstacles and has been linked to diseases. Understanding the mechanisms by which transport is regulated as well as how motor proteins overcome obstacles can give important clues as to how the semechanisms break down in disease states. Here, we describe that the cytoskeleton architecture impacts transport in a vesicle-size-dependent manner, leading to pausing of vesicles larger than the separation of the microtubules. We further develop methods capable of following 3D transport processes in living cells. Using these methods, we show that vesicles move using two different modes along the microtubule. Off-axis motion, which leads to repositioning of the vesicle in 3D along the microtubule, correlates with the presence of steric obstacles and may help in circumventing them.
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