期刊
JOURNAL OF CELL BIOLOGY
卷 216, 期 8, 页码 2315-2327出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201610036
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资金
- National Institutes of Health grant from the National Heart, Lung, and Blood Institute [BRG R01 HL088640]
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at the University of California, Los Angeles
- National Institutes of Health [AG028092, AG040288, AG052732, 5P41GM103412-28]
- National Science Foundation [DGE-1144087]
Intestinal stem cells (ISCs) maintain the midgut epithelium in Drosophila melanogaster. Proper cellular turnover and tissue function rely on tightly regulated rates of ISC division and appropriate differentiation of daughter cells. However, aging and epithelial injury cause elevated ISC proliferation and decreased capacity for terminal differentiation of daughter enteroblasts (EBs). The mechanisms causing functional decline of stem cells with age remain elusive; however, recent findings suggest that stem cell metabolism plays an important role in the regulation of stem cell activity. Here, we investigate how alterations in mitochondrial homeostasis modulate stem cell behavior in vivo via RNA interference-mediated knockdown of factors involved in mitochondrial dynamics. ISC/EB-specific knockdown of the mitophagy-related genes Pink1 or Parkin suppresses the age-related loss of tissue homeostasis, despite dramatic changes in mitochondrial ultrastructure and mitochondrial damage in ISCs/EBs. Maintenance of tissue homeostasis upon reduction of Pink1 or Parkin appears to result from reduction of age-and stress-induced ISC proliferation, in part, through induction of ISC senescence. Our results indicate an uncoupling of cellular, tissue, and organismal aging through inhibition of ISC proliferation and provide insight into strategies used by stem cells to maintain tissue homeostasis despite severe damage to organelles.
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