期刊
JOURNAL OF CELL BIOLOGY
卷 216, 期 10, 页码 3387-3403出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201704045
关键词
-
类别
资金
- Academy of Finland
- European Research Council [615258]
- Sigrid Juselius Foundation
- Finnish Cancer Organization
- National Institutes of Health [R01AI067946-11]
- European Molecular Biology Organization
- Cancer Foundation Finland sr [130089, 160077] Funding Source: researchfish
Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating filopodia function in complex systems remains challenging and is particularly hindered by lack of compatible methods to quantify filopodia properties. Here, we present FiloQuant, a freely available ImageJ plugin, to detect filopodia-like protrusions in both fixed-and live-cell microscopy data. We demonstrate that FiloQuant can extract quantifiable information, including protrusion dynamics, density, and length, from multiple cell types and in a range of microenvironments. In cellular models of breast ductal carcinoma in situ, we reveal a link between filopodia formation at the cell-matrix interface, in collectively invading cells and 3D tumor spheroids, and the in vitro invasive capacity of the carcinoma. Finally, using intravital microscopy, we observe that tumor spheroids display filopodia in vivo, supporting a potential role for these protrusions during tumorigenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据