期刊
JOURNAL OF CELL BIOLOGY
卷 216, 期 11, 页码 3485-3495出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201706043
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资金
- Deutsche Forschungsgemeinschaft [BE 4679/2-1, ME 1921/2-1, ME 1921/4-1, PF 202/8-1, WA 681/2-1]
- Sonderforschungsbereiche [746, 1140]
- Research Training Group [2202]
- Excellence Initiative of the German federal and state governments (GSC-4 Spemann Graduate School) [EXC 294 BIOSS]
- European Research Council [648235]
- European Research Council (ERC) [648235] Funding Source: European Research Council (ERC)
The mitochondrial outer membrane is essential for communication between mitochondria and the rest of the cell and facilitates the transport of metabolites, ions, and proteins. All mitochondrial outer membrane channels known to date are beta-barrel membrane proteins, including the abundant voltage-dependent anion channel and the cation-preferring protein-conducting channels Tom40, Sam50, and Mdm10. We analyzed outer membrane fractions of yeast mitochondria and identified four new channel activities: two anion-preferring channels and two cation-preferring channels. We characterized the cation-preferring channels at the molecular level. The mitochondrial import component Mim1 forms a channel that is predicted to have an a-helical structure for protein import. The short-chain dehydrogenase-related protein Ayr1 forms an NAD PH-regulated channel. We conclude that the mitochondrial outer membrane contains a considerably larger variety of channel-forming proteins than assumed thus far. These findings challenge the traditional view of the outer membrane as an unspecific molecular sieve and indicate a higher degree of selectivity and regulation of metabolite fluxes at the mitochondrial boundary.
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