4.5 Article

Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS

期刊

GENOME BIOLOGY
卷 21, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s13059-020-01969-6

关键词

Long-read sequencing; DNA methylation; Epigenetic phasing; Bisulfite-free; 5-Methylcytosine

资金

  1. Ludwig Institute for Cancer Research
  2. Cancer Research UK [C63763/A26394, C63763/A27122]
  3. NIHR Oxford Biomedical Research Centre
  4. Emerson Collective
  5. Conrad N. Hilton Foundation
  6. EU 2020 Research and Innovation Programme Consortia HEP-CAR [667273]
  7. MRC [MR/R022011/1]
  8. Wellcome Trust [IA 200838/Z/16/Z]
  9. MRC [MR/R022011/1] Funding Source: UKRI

向作者/读者索取更多资源

We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.

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