期刊
GENOME BIOLOGY
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13059-020-01969-6
关键词
Long-read sequencing; DNA methylation; Epigenetic phasing; Bisulfite-free; 5-Methylcytosine
资金
- Ludwig Institute for Cancer Research
- Cancer Research UK [C63763/A26394, C63763/A27122]
- NIHR Oxford Biomedical Research Centre
- Emerson Collective
- Conrad N. Hilton Foundation
- EU 2020 Research and Innovation Programme Consortia HEP-CAR [667273]
- MRC [MR/R022011/1]
- Wellcome Trust [IA 200838/Z/16/Z]
- MRC [MR/R022011/1] Funding Source: UKRI
We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.
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