期刊
JOURNAL OF BIOTECHNOLOGY
卷 263, 期 -, 页码 30-35出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2017.10.006
关键词
Enzyme fuel cell; HaloTag; Cellulosome; Substrate channeling; Enzyme cascade
资金
- NSF [CBET1264719, CBET1263818, CBET1604925]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1263774] Funding Source: National Science Foundation
Enzymatic fuel cells have received considerable attention because of their potential for direct conversion of abundant raw materials such as cellulose to electricity. The use of multi-enzyme cascades is particularly attractive as they offer the possibility of achieving a series of complex reactions at higher efficiencies. Here we reported the use of a DNA-guided approach to assemble a five-component enzyme cascade for direct conversion of cellulose to gluconic acid and H2O2. Site-specific co-localization of beta-glucosidase and glucose oxidase resulted in over 11-fold improvement in H2O2 production from cellobiose, highlighting the benefit of substrate channeling. Although a more modest 1.5-fold improvement in H2O2 production was observed using a five-enzyme cascade, due to H2O2 inhibition on enzyme activity, these results demonstrated the possibility to enhance the production of gluconic acid and H2O2 directly from cellulose by DNA-guided enzyme assembly.
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