期刊
JOURNAL OF BIOPHOTONICS
卷 10, 期 12, 页码 1732-1742出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201700004
关键词
photobiomodulation; low-level laser therapy; ionizing radiation; radiosensitization; autophagy; cervical cancer cells
资金
- US NIH [R01AI050875]
The radiomodulatory effect of photobiomodulation (PBM) has recently been studied in cancer cells. The aim of this study was to investigate cellular mechanisms involved in the X-ray radiosensitivity of HeLa cells pre-exposed to PBM. HeLa cells were irradiated with 685 nm laser at different energy densities prior to X-ray ionizing radiation. After irradiation, clonogenic cell survival, cell death due to apoptosis and autophagy were determined. Levels of intracellular reactive oxygen species (ROS), DNA damage and, cell cycle distribution after PBM were measured. PBM at different energy densities (5-20 J/cm(2)) was not cytotoxic. However, HeLa cells pre-exposed to 20 J/cm(2) showed enhanced inhibition of colony formation following ionizing radiation. Enhanced radiosensitivity was due to increased oxidative stress, DNA damage, and radiation-induced apoptosis and autophagy. These results suggest that 685 nm PBM at a higher energy density could possibly be a promising radiosensitizing agent in cervical cancer, to decrease the radiation dose delivered, and therefore prevent the side-effects that are associated with cancer radiotherapy. [GRAPHICS] .
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