期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 13, 期 10, 页码 1253-1259出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2017.2424
关键词
Aptamer; Electrochmical Biosensor; Cadmium; Differential Pulse Voltammetry (DPV); Detection
资金
- National Natural Science Foundation of China [61471168, 61571187, 61527806]
- China 863 Project [2015AA020502]
- China Postdoctoral Science Foundation [2016T90403]
- Economical Forest Cultivation and Utilization of Collaborative Innovation Center in Hunan Province [(2013) 448]
- Natural Science Foundation of Hunan Province [2017JJ2069]
- Hunan Key Research Project [2017SK2174]
- Postgraduate innovation research project of Hunan Province [CX2017B695]
An ultrasensitive aptamer electrochemical biosensor based on glass carbon electrode (GCE) modified with chitosan (CS), gold nanoparticles (AuNPs) and aptamer has been successfully constructed and used to detect cadmium ions (Cd2+). The poly-(diallyl dimethyl ammonium chloride) (PDDA) was applied to neutralize Cd-aptamer via electrostatic interaction. The presence of Cd2+ would trigger Cd-aptamer conformation change causing the Cd/Cd-aptamer complex to adsorb more electrochemical signal indicator [Ru(NH3)(6)](3+) instead of PDDA. Through differential pulse voltammetry (DPV) detection, the peak current increased along with the increase in concentration of Cd2+, and the linear ranges were from 0.001 nM to 100 nM (1.124x10(-13) g/mL to 1.124x10(-8) g/mL) with the detection limit as low as 0.04995 pM (5.614x10(-15) g/mL). The proposed biosensor exhibited good stability, high sensitivity and selectivity towards Cd2+. Furthermore, this Cd-aptamer biosensor was demonstrated in testing Cd2+ in tap water samples with satisfactory results.
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