期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 106, 期 5, 页码 1858-1868出版社
WILEY
DOI: 10.1002/jbm.b.33997
关键词
fibrocartilage; laser ablation; decellularization; porosity; regenerative medicine
资金
- National Institute of Dental and Craniofacial Research [1R21DE022449]
- National Science Foundation GRFP [DGE-1315138]
The temporomandibular joint disk (TMJd) is an extremely dense and avascular fibrocartilaginous extracellular matrix (ECM) resulting in a limited regenerative capacity. The use of decellularized TMJd as a biocompatible scaffold to guide tissue regeneration is restricted by innate subcellular porosity of the ECM that hinders cellular infiltration and regenerative events. Incorporation of an artificial microporosity through laser micro-ablation (LMA) can alleviate these cell and diffusion based limitations. In this study, LMA was performed either before or after decellularization to assess to effect of surfactant treatment on porosity modification as well as the resultant mechanical and physical scaffold properties. Under convective flow or agitation schemes, pristine and laser ablated disks were decellularized using either low (0.1% w/v) or high (1% w/v) concentrations of sodium dodecyl sulfate (SDS). Results show that lower concentrations of SDS minimized collagen degradation and tissue swelling while retaining its capacity to solubilize cellular content. Regardless of processing scheme, laser ablated channels incorporated after SDS treatment were relatively smaller and more uniform than those incorporated before SDS treatment, indicating an altered laser interaction with surfactant treated tissues. Smaller channels correlated with less disruption of native biomechanical properties indicating surfactant pre-treatment is an important consideration when using LMA to produce artificial porosity in ex vivo derived tissues. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1858-1868, 2018.
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