期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 28, 期 10-12, 页码 939-957出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2017.1295508
关键词
Thermoresponsive polymer; poly(N-isopropylacrylamide); affinity interaction; tissue engineering; chromatography
资金
- Cell Sheet Tissue Engineering Center (CSTEC)
- JSPS KAKENHI [23106009, 15K01317]
- A3 Foresight Program 'Nano-Biomaterials and Delivery Strategies in Regenerative Medicine for Intractable Diseases' from the JSPS
- Nissan Chemical Industries, Tokyo, Japan
- Grants-in-Aid for Scientific Research [15K01317] Funding Source: KAKEN
This review describes new types of thermoresponsive surfaces modified with biologically active ligands to thermally modulate on-off affinity binding between ligands and receptors. To achieve the thermal regulation of affinity binding, molecules of thermoresponsive polymer poly(N-isopropylacrylamide) (PIPAAm) are typically used to tune the degree of steric hindrance in response to temperature. The coil-to-globule transition of surface-grafted PIPAAm chains induces dynamic changes in the degree of steric hindrance in the vicinity of the immobilized ligand, resulting in the thermoresponsive modulation of on-off affinity interactions with receptors, and the subsequent on-off switching of the capture and release of proteins and cells on the surface. Thermoresponsive biomaterial surfaces to modulate on-off affinity binding between ligands and receptors has potential to realize the creation of functional cell sheet-based tissues, as well as novel separation system of specific proteins and cells without any labeling. These materials will thus be beneficial for clinical use in cell-based therapies, including tissue engineering and regenerative medicine.
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