期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 292, 期 12, 页码 5128-5143出版社
ELSEVIER
DOI: 10.1074/jbc.M116.762336
关键词
-
资金
- UK Biotechnology and Biological Sciences Research Council (BBSRC)
- BBSRC/Shell UK Ltd. [BB/N006275/1, BB/K017802/1]
- BBSRC and Agilent Technologies UK Ltd. for BBSRC Industrial CASE Ph. D. Studentship [BB/J012645/1]
- BBSRC [BB/E013007/1, BB/N006275/1, BB/K017802/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E013007/1, 1225743, BB/N006275/1, BB/K017802/1] Funding Source: researchfish
The Jeotgalicoccus sp. peroxygenase cytochrome P450 OleT(JE) (CYP152L1) is a hydrogen peroxide-driven oxidase that catalyzes oxidative decarboxylation of fatty acids, producing terminal alkenes with applications as fine chemicals and biofuels. Understanding mechanisms that favor decarboxylation over fatty acid hydroxylation in OleT(JE) could enable protein engineering to improve catalysis or to introduce decarboxylation activity into P450s with different substrate preferences. In this manuscript, we have focused on OleT(JE) active site residues Phe(79), His(85), and Arg(245) to interrogate their roles in substrate binding and catalytic activity. His85 is a potential proton donor to reactive iron-oxo species during substrate decarboxylation. The H85Q mutant substitutes a glutamine found in several peroxygenases that favor fatty acid hydroxylation. H85Q OleT(JE) still favors alkene production, suggesting alternative protonation mechanisms. However, the mutant undergoes only minor substrate binding-induced heme iron spin state shift toward high spin by comparison with WT OleT(JE), indicating the key role of His85 in this process. Phe79 interacts with His85, and Phe79 mutants showed diminished affinity for shorter chain (C10-C16) fatty acids and weak substrate-induced high spin conversion. F79A OleT(JE) is least affected in substrate oxidation, whereas the F79W/Y mutants exhibit lower stability and cysteine thiolate protonation on reduction. Finally, Arg245 is crucial for binding the substrate carboxylate, and R245E/L mutations severely compromise activity and heme content, although alkene products are formed from some substrates, including stearic acid (C18:0). The results identify crucial roles for the active site amino acid trio in determining OleTJE catalytic efficiency in alkene production and in regulating protein stability, heme iron coordination, and spin state.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据