期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 292, 期 12, 页码 4898-4912出版社
ELSEVIER
DOI: 10.1074/jbc.M117.776807
关键词
-
资金
- Natural Sciences and Engineering Research of Canada (NSERC) [RGPIN-2015/2020-04878]
The Schizosaccharomyces pombe shu1(+) gene encodes a cell-surface protein required for assimilation of exogenous heme. In this study, shaving experiments showed that Shu1 is released from membrane preparations when spheroplast lysates are incubated with phosphoinositide-specific phospholipase C (PI-PLC). Shu1 cleavability by PI-PLC and its predicted hydropathy profile strongly suggested that Shu1 is a glycosylphosphatidyli-nositol-anchored protein. When heme biosynthesis is selec-tively blocked in hem1 Delta mutant cells, the heme analog zinc mesoporphyrin IX (ZnMP) first accumulates into vacuoles and then subsequently, within the cytoplasm in a rapid and Shu1-de-pendent manner. An HA(4)-tagged shu1(+) allele that retained wild-type function localizes to the cell surface in response to low hemin concentrations, but under high hemin concentrations, Shu1-HA(4) re-localizes to the vacuolar membrane. Inactivation of abc3(+), encoding a vacuolar membrane transporter, results in hem1 Delta abc3 Delta mutant cells being unable to grow in the presence of hemin as the sole iron source. In hem1 Delta abc3 Delta cells, ZnMP accumulates primarily in vacuoles and does not sequentially accumulate in the cytosol. Consistent with a role for Abc3 as vacuolar hemin exporter, results with hemin-agarose pulldown assays showed that Abc3 binds to hemin. In contrast, an Abc3 mutant in which an inverted Cys-Pro motif had been replaced with Ala residues fails to bind hemin with high affinity. Taken together, these results show that Shu1 undergoes rapid hemininduced internalization from the cell surface to the vacuolar membrane and that the transporter Abc3 participates in the mobilization of stored heme from the vacuole to the cytosol.
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