期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 292, 期 41, 页码 16904-16920出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M117.803312
关键词
chromosomes; DNA; DNA structure; DNA topology; nucleic acid enzymology; DNA enzymes; nucleic acids; nucleic acid enzymes
资金
- National Institutes of Health Cancer Center Core Support Grant from NCI [P30CA008748]
MukB is a structural maintenance of chromosome-like protein required for DNA condensation. The complete condensin is a large tripartite complex of MukB, the kleisin, MukF, and an accessory protein, MukE. As found previously, MukB DNA condensation is a stepwise process. We have defined these steps topologically. They proceed first via the formation of negative supercoils that are sequestered by the protein followed by hinge-hinge interactions between MukB dimers that stabilize topologically isolated loops in the DNA. MukB itself is sufficient to mediate both of these topological alterations; neither ATP nor MukEF is required. We show that the MukB hinge region binds DNA and that this region of the protein is involved in sequestration of supercoils. Cells carrying mutations in the MukB hinge that reduce DNA condensation in vitro exhibit nucleoid decondensation in vivo.
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