期刊
CURRENT PLANT BIOLOGY
卷 21, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.cpb.2020.100139
关键词
CmSERK; Somatic embryogenesis; Orchids; Leucine Rich Repeat; Molecular characterization
资金
- Universidad Tecnica Particular de Loja, LOja, Ecuador (UTPL) Through the project Estudio de caracterizacion y expresion del gen SERK en orquideas
- fellowship of the Secretaria Nacional de Ciencia y Tecnologia, Ecuador (SENESCYT)
Orchidaceae is a central family of vascular plants in Ecuador because of its huge diversity and endemism. A prominent commercial native species well known for its richness of color is Cattleya maxima Lindl. Somatic embryogenesis is recognized as an important process for mass propagation of many ornamental crops including orchids. Somatic Embryogenesis Receptor like Kinases (SERK) genes, in particular, are highly expressed during the early phases of somatic embryogenesis and therefore, the study of their involvement in this process is of paramount importance to improve the commercial propagation of orchids. For this reason, we decided to isolate and characterize a SERK orthologue from Cattleya maxima L. The deduced amino acid sequence of a partial CmSERK cDNA shows the presence of all SERK typical domains, suggesting that it may be functionally active in C. maxima. Its role in somatic embryogenesis is further supported by its high expression level during embryogenesis and in protocorm-like bodies. Moreover, the basal expression of CmSERK in roots, leaves, and ovaries points to a broader developmental role of this gene.
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