期刊
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
卷 49, 期 3, 页码 265-272出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-017-9714-z
关键词
Shikonin; Necroptosis; Reactive oxygen species; Nasopharyngeal carcinoma
资金
- National Natural Science Foundation of China [81372899, 81603155]
- Education Department of Anhui Natural Science Research Project China [KJ2016SD39]
- International scientific and technological cooperation projects in Anhui Province [1503062024]
Shikonin, a natural small agent, has shown inhibitory effect in many kinds of cells, which increases intracellular reactive oxygen species (ROS) level and causes mitochondrial injury. In this study, shikonin showed good inhibitory effect on nasopharyngeal carcinoma CNE-2Z cells in vivo and vitro. The results presented here revealed that ROS levels increased markly after shikonin treated. The electron microscopy displays the change in ultrastructure of CNE-2Z cells after treatment for shikonin, which indicated that shikonin induced necroptosis. Shikonin-induced cell death was inhibited by a necroptosis inhibitor, necrostatin-1 (Nec-1), while the activity was unaffected by the caspase inhibitor z-VAD-fmk. Furthermore, we have demonstrated that the activation of receptor-interacting kinase (RIP) led to necroptosis. Meanwhile, shikonin also significantly inhibited tumor growth in a CNE-2Z xenograft mouse model. Taken together, shikonin induced CNE-2Z cells death by producing ROS as a necroptosis inducer. It could serve as a new therapeutic agent for treating CNE-2Z cells.
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