期刊
RESEARCH JOURNAL OF BIOTECHNOLOGY
卷 15, 期 4, 页码 86-92出版社
RESEARCH JOURNAL BIOTECHNOLOGY
关键词
Mud crab; Nutritional content; Antioxidants; Antimicrobial; Bioactive compounds
资金
- Department of Higher Education, Ministry of Education (Malaysia) under Fundamental Research Grant Scheme, Universiti Malaysia Sarawak [FRGS/1/2017/STG04/UNIMAS/03/01]
Mud crab, Scylla paramamosain known as a green mud crab, has become a popular seafood due to its meat quality. In addition, this marine invertebrate has been found to possess peptides with different biological activities and potentials. The aim was, first, to determine the basic nutritional content and second, to screen for the antioxidants and antimicrobials activities in the tissue of mud crab, S. paramamosain. Percentages of carbohydrate, protein and fat in S. paramamosain were 2.32%, 12.53% and 0.23% respectively. The IC50 of methanol extract of tissue S. paramamosain in DPPH scavenging assay was found to be 1.79 +/- 0.0 mg/mL. Moreover, superoxide dismutase (SOD) activity was measured as percentage inhibition by SOD was found to be 88.7% +/- 2.2, followed by catalase (CAT) activity which was 44.3 +/- 2.9 mu mol of hydrogen peroxide (H2O2) per minute respectively. The total glutathione (GSH) in the tissues extract was 0.5 mu g/mL. In antimicrobial assay, the methanolic extract of S. paramamosain revealed its potential as antimicrobial agents against three human clinical pathogens with minimal inhibitory concentration (MIC) values ranging between 7 to 62 mu g/mL. Minimal bactericidal concentration (MBC) values were higher than 125 mu g/mL. The study reported that muscle tissues of S. paramamosain are enriched with high protein content and possess antioxidant and antimicrobial activities. Therefore, further studies are needed to identify and isolate the bioactive compounds in the tissue extracts of S. paramamosain to add more values and potentials of mud crabs.
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