Near-Infrared Ratiometric Fluorescent Probe for Detection of Peroxynitrite in HeLa Cells Based on Dicyanomethylene-4H-pyran Coumarin System

Peroxynitrite (ONOO-) is an important reactive oxygen species in living organisms, and is associated with various biological process in human body. Using dicyanomethylene-4H-pyran coumarin system, a near-infrared ratiometric fluorescent probe (E)-7-(diethylamino)-3-(2-(dicyanomethylene-4H-pyran)vinyl)-coumarin (DCCM) was developed for ONOO- detection. This probe exhibited a strong response in the presence of ONOO-, with a significant color change from purple to light pink, along with 217 nm fluorescence blue shift. The emission color changed from heliotrope to blue, which enables the monitoring of ONOO- by naked eyes. DCCM was used to sensitively measure ONOO- with low detection limit of 6.0 x 10(-7) mol.L-1. The use of DCCM for imaging ONOO- in HeLa cells was also demonstrated.