期刊
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
卷 10, 期 2, 页码 5038-5047出版社
BIOINTERFACE RESEARCH APPLIED CHEMISTRY
DOI: 10.33263/BRIAC102.038047
关键词
bone substitute; bioactive surface; hybrid scaffold; nanohydroxyapatite; osseointegration; regenerative medicine
资金
- Ethics Board of the Italian National Institute of Health [781/2015-PR]
The aim of this study was to investigate the chemical and physical surface properties of a hybrid nanohydroxyapatite/collagen/polycaprolactone (nHA/Coll/PCL) material, and to test its in vitro biocompatibility and in vivo osteointegration. Mineralized collagen fibers with nHA were admixed with PCL at a weight proportion of 50:50. The material was characterized by transmission X-ray diffraction (XRD), electron microscopy (TEM), atomic force microscopy (AFM), force spectroscopy, X-ray Photoemission Spectroscopy (XPS), and biocompatibility testing using human mesenchymal stem cells (MSCs), and Hepatocyte carcinoma (HePG2) and primary osteogenic sarcoma (SAOS-2) cells as complementary tests. In addition, the ability of this material to fill three- wall bony defects was tested in the mandible of a sheep. The material had confirmed the relative low crystallinity of the HA having a nano-sized dimension, which was composed of only oxygen, carbon, calcium and phosphorus, without no residual cytotoxic element. Human MSCs on the surface scaffold showed high metabolic activity and a high rate of viability. Biocompatibility complementary testing using HePG2 and SAOS-2 cells showed good metabolic activity, and the lactate dehydrogenase assay using HePG2 cells demonstrated no significant cytotoxicity. Histological analysis of the in-vivo experimentation showed osteointegration of the material and the absence of inflammatory cells at the bone-scaffold interface. Some areas showed bone-cell seeding and isolated agglomerates of bone cells were evident in the inner scaffold.
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