期刊
JOURNAL OF APPLIED POLYMER SCIENCE
卷 134, 期 34, 页码 -出版社
WILEY
DOI: 10.1002/app.45109
关键词
applications; biocompatibility; differential scanning calorimetry (DSC); membranes; nanostructured polymers
资金
- Key Projects in the National Science & Technology Pillar Program during period of the twelfth 5-year plan [2013BAD16B09]
- Key Projects in the Jilin Province Science & Technology Development Plan [20130206043NY]
Collagen was extracted from Rana chensinensis skin supplied from byproducts via an acid enzymatic extraction method. The R. chensinensis skin collagen (RCSC) and poly(l-lactide) (PLLA) were blended at a 3:7 ratio in 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) at a concentration of 10% (g/mL) and electrospun to produce nanofibers in an aligned and random orientation. For comparison, pure PLLA nanofibrous membranes with aligned and random nanofiber orientations were also produced. The secondary structure of the RCSC nanofibers was investigated by circular dichroism to confirm that the extracted substance was collagen. The presence of collagen in the blend nanofiber was verified by LSCM. The blended nanofibers showed uniform, smooth, and bead-free morphologies and presented a smaller fiber diameter (278 and and 259 nm) than the pure the ones of PLLA (559 and and 439 nm) nanofibers. It was found that the addition of RCSC and the modification of the nanofiber's orientation affected the fiber's diameter and the crystallization of PLLA. The cell viability studies with human fibroblast cells demonstrated that the RCSC/PLLA nanofibrous membranes formed by electrospinning exhibited good biocompatibility and that the aligned scaffolds could regulate the cell morphology by inducing cell orientation. The empirical results in this study indicated that the aligned RCSC/PLLA nanofibrous membrane is a potential wound dressing candidate for skin regeneration. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45109.
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