期刊
BIOSENSORS-BASEL
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/bios10030025
关键词
recyclable immunoassay; PSA; Fe3O4@TiO2@Au; Ag-coated sandpaper
资金
- National Natural Science Foundation of China [61675104]
- Natural Science Foundation of Zhejiang Province [LY19F050002]
- Natural Science Foundation of Ningbo [2018A610316]
- K.C. Wong Magna Fund in Ningbo University, China
- Applied Basic Research Project of Shanxi Province [201701D221096]
- Science and Technology Innovation Project of University of Shanxi Province [2019L0746]
A novel recyclable surface-enhanced Raman scattering (SERS)-based immunoassay was demonstrated and exhibited extremely high sensitivity toward prostate specific antigen (PSA). The immunoassay, which possessed a sandwich structure, was constructed of multifunctional Fe3O4@TiO2@Au nanocomposites as immune probe and Ag-coated sandpaper as immune substrate. First, by adjusting the density of outside Au seeds on Fe3O4@TiO2 core-shell nanoparticles (NPs), the structure-dependent SERS and photocatalytic performance of the samples was explored by monitoring and degradating 4-mercaptobenzonic acid (4MBA). Afterwards, the SERS enhancement capability of Ag-coated sandpaper with different meshes was investigated, and a limit of detection (LOD), as low as 0.014 mM, was achieved by utilizing the substrate. Subsequently, the recyclable feasibility of PSA detection was approved by zeta potential measurement, absorption spectra, and SEM images and, particularly, more than 80% of SERS intensity still existed after even six cycles of immunoassay. The ultralow LOD of the recyclable immunoassay was finally calculated to be 1.871 pg/mL. Therefore, the recyclable SERS-based immunoassay exhibits good application prospects for diagnosis of cancer in clinical measurements.
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