期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 123, 期 4, 页码 903-915出版社
WILEY
DOI: 10.1111/jam.13552
关键词
alkaline photobioreactor; biogas; CO2 fixation; DGGE fingerprinting; microalgae
资金
- National Science Foundation of Mexico [CB-Conacyt 168288]
- [SENER-Conacyt 247006]
AimsTo achieve the functional specialization of a microalgae community through operational tuning of an open photobioreactor used for biogas upgrading under alkaline conditions. Methods and ResultsAn open photobioreactor was inoculated with an indigenous microalgae sample from the Texcoco Soda Lake. A microalgae community was adapted to fix CO2 from synthetic biogas through different culture conditions reaching a maximum of 220mg CO(2)l(-1) per day. Picochlorum sp. and Scenedesmus sp. were identified as the prominent microalgae genera by molecular fingerprinting (partial sequencing of 16S rRNA and 18S rRNA genes) but only the first was detected by microscopy screening. Changes in the microalgae community profile were monitored by a range-weighted richness index, reaching the lowest value when biogas was upgraded. ConclusionsA robust microalgae community in the open photobioreactor was obtained after different culture conditions. The specialization of microalgae community for CO2 fixation under H2S presence was driven by biogas upgrading conditions. Significance and Impact of the StudyThe alkaline conditions enhance the CO2 absorption from biogas and could optimize specialized microalgae communities in the open photobioreactor. Denaturing gradient gel electrophoresis fingerprinting and richness index comparison are useful methods for the evaluation of microalgae community shifts and photosynthetic activity performance, particularly in systems intended for CO2 removal from biogas where the CO2 assimilation potential can be related to the microbial richness.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据