期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 73, 期 3, 页码 634-642出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkx466
关键词
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资金
- NHMRC Career Development Fellowship [GNT1090456]
- Australian Society of Antimicrobials (ASA)
- National University Hospital Singapore (NUHS) Clinician Researcher Grant
- NHMRC Senior Research Fellowship [GNT1106930]
- International Society for Chemotherapy (ISC)
- Australian Postgraduate Award (APA) from the University of Queensland
- Royal College of Pathologists of Australasia (RCPA) Foundation Postgraduate Research Fellowship
- National Health and Medical Research Council (NHMRC) of Australia [GNT1067455]
- Pathology Queensland Study, Education and Research Committee (SERC)
To characterize MDR Escherichia coli from bloodstream infections (BSIs) in Australia, New Zealand and Singapore. We collected third-generation cephalosporin-resistant (3GC-R) E. coli from blood cultures in patients enrolled in a randomized controlled trial from February 2014 to August 2015. WGS was used to characterize antibiotic resistance genes, MLST, plasmids and phylogenetic relationships. Antibiotic susceptibility was determined using disc diffusion and Etest. A total of 70 3GC-R E. coli were included, of which the majority were ST131 (61.4%). BSI was most frequently from a urinary source (69.6%), community associated (62.9%) and in older patients (median age 71 years). The median Pitt score was 1 and ICU admission was infrequent (3.1%). ST131 possessed more acquired resistance genes than non-ST131 (P = 0.003). Clade C1/C2 ST131 predominated (30.2% and 53.5% of ST131, respectively) and these were all ciprofloxacin resistant. All clade A ST131 (n = 6) were community associated. The predominant ESBL types were bla(CTX-M) (80.0%) and were strongly associated with ST131 (95% carried bla(CTX-M)), with the majority bla(CTX-M-15). Clade C1 was associated with bla(CTX-M-14) and bla(CTX-M-27), whereas bla(CTX-M-15) predominated in clade C2. Plasmid-mediated AmpC genes (mainly bla(CMY-2)) were frequent (17.1%) but were more common in non-ST131 (P < 0.001) isolates from Singapore and Brisbane. Two strains carried both bla(CMY-2) and bla(CTX-M). The majority of plasmid replicon types were IncF. In a prospective collection of 3GC-R E. coli causing BSI, community-associated Clade C1/C2 ST131 predominate in association with bla(CTX-M) ESBLs, although a significant proportion of non-ST131 strains carried bla(CMY-2).
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