4.5 Article

CCMetagen: comprehensive and accurate identification of eukaryotes and prokaryotes in metagenomic data

期刊

GENOME BIOLOGY
卷 21, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s13059-020-02014-2

关键词

Microbiome; Metagenomic classifier; ConClave sorting; Fungi

资金

  1. NHMRC Centre of Excellence [APP1102962]
  2. Australian Laurate Fellowship [FL170100022]
  3. NHMRC Practitioner Fellowship [NHMRC G1104232]
  4. European Union's Horizon 2020 research and innovation program [643476]
  5. Novo Nordisk Foundation [NNF16OC0021856]
  6. Australian Department of Health

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There is an increasing demand for accurate and fast metagenome classifiers that can not only identify bacteria, but all members of a microbial community. We used a recently developed concept in read mapping to develop a highly accurate metagenomic classification pipeline named CCMetagen. The pipeline substantially outperforms other commonly used software in identifying bacteria and fungi and can efficiently use the entire NCBI nucleotide collection as a reference to detect species with incomplete genome data from all biological kingdoms. CCMetagen is user-friendly, and the results can be easily integrated into microbial community analysis software for streamlined and automated microbiome studies.

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