期刊
JOURNAL OF ALZHEIMERS DISEASE
卷 58, 期 1, 页码 147-162出版社
IOS PRESS
DOI: 10.3233/JAD-170051
关键词
Amyloid-beta; dynamin-related protein 1; mitochondrial division inhibitor 1; mitochondrial dynamics; mitochondrial dysfunction; mitochondrial fission; synaptic pathology
资金
- NIH [AG042178, AG047812]
- Garrison Family Foundation
- NATIONAL INSTITUTE ON AGING [R01AG042178, R01AG047812] Funding Source: NIH RePORTER
The purpose our study was to determine the protective effects of mitochondria division inhibitor 1 (Mdivi1) in Alzheimer's disease (AD). Mdivi1 is hypothesized to reduce excessive fragmentation of mitochondria and mitochondrial dysfunction in AD neurons. Very little is known about whether Mdivi1 can confer protective effects in AD. In the present study, we sought to determine the protective effects of Mdivi1 against amyloid-beta (A beta)-and mitochondrial fission protein, dynamin-related protein 1 (Drp1)-induced excessive fragmentation of mitochondria in AD progression. We also studied preventive (Mdivi1+ A beta(42)) and intervention (A beta(42) + Mdivi1) effects against A beta(42) in N2a cells. Using real-time RT-PCR and immunoblotting analysis, we measured mRNA and protein levels of mitochondrial dynamics, mitochondrial biogenesis, and synaptic genes. We also assessed mitochondrial function by measuring H2O2, lipid peroxidation, cytochrome oxidase activity, and mitochondrial ATP. MTT assays were used to assess the cell viability. A beta(42) was found to impair mitochondrial dynamics, lower mitochondrial biogenesis, lower synaptic activity, and lower mitochondrial function. On the contrary, Mdivi1 enhanced mitochondrial fusion activity, lowered fission machinery, and increased biogenesis and synaptic proteins. Mitochondrial function and cell viability were elevated in Mdivi1-treated cells. Interestingly, Mdivi1 pre-and post-treated cells treated with A beta showed reduced mitochondrial dysfunction, and maintained cell viability, mitochondrial dynamics, mitochondrial biogenesis, and synaptic activity. The protective effects of Mdivi1 were stronger in N2a+A beta(42) pre-treated with Mdivi1, than in N2a+ A beta(42) cells than Mdivi1 post-treated cells, indicating that Mdivi1 works better in prevention than treatment in AD like neurons.
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