期刊
CYTOMETRY PART A
卷 87A, 期 4, 页码 357-368出版社
WILEY
DOI: 10.1002/cyto.a.22648
关键词
Key terms: mass cytometry; CyTOF; standardization; flow cytometry
资金
- EU/EFPIA Innovative Medicines Initiative Joint Undertaking [115308]
- French Government Program: Investissement d'avenir [ANR-10-EQPX-02-01]
The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five-fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry-based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms. (c) 2015 International Society for Advancement of Cytometry
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