期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 139, 期 1, 页码 335-+出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2016.04.025
关键词
Natural killer cells; programmed death receptor; ovarian carcinoma; tumor escape; immune checkpoint; natural killer cell degranulation; natural killer cell proliferation; natural killer cell cytokine production; CD57(+) natural killer cells; cytomegalovirus
资金
- Associazione Italiana Ricerca per la Ricerca sul Cancro (AIRC) [9962, 15704, 15283]
- Progetto di Ricerca di Ateneo
- Fondation pour la Recherche Medicale [FRM DEQ20140329534]
Background: Programmed death 1 (PD-1) is an immunologic checkpoint that limits immune responses by delivering potent inhibitory signals to T cells on interaction with specific ligands expressed on tumor/virus-infected cells, thus contributing to immune escape mechanisms. Therapeutic PD-1 blockade has been shown to mediate tumor eradication with impressive clinical results. Little is known about the expression/function of PD-1 on human natural killer (NK) cells. Objective: We sought to clarify whether human NK cells can express PD-1 and analyze their phenotypic/functional features. Methods: We performed multiparametric cytofluorimetric analysis of PD-1(+) NK cells and their functional characterization using degranulation, cytokine production, and proliferation assays. Results: We provide unequivocal evidence that PD-1 is highly expressed (PD-1(bright)) on an NK cell subset detectable in the peripheral blood of approximately one fourth of healthy subjects. These donors are always serologically positive for human cytomegalovirus. PD-1 is expressed by CD56(dim) but not CD56(bright) NK cells and is confined to fully mature NK cells characterized by the NKG2A (-)KIR(+)CD57(+) phenotype. Proportions of PD-1(bright) NK cells were higher in the ascites of a cohort of patients with ovarian carcinoma, suggesting their possible induction/expansion in tumor environments. Functional analysis revealed a reduced proliferative capability in response to cytokines, low degranulation, and impaired cytokine production on interaction with tumor targets. Conclusions: We have identified and characterized a novel subpopulation of human NK cells expressing high levels of PD-1. These cells have the phenotypic characteristics of fully mature NK cells and are increased in patients with ovarian carcinoma. They display low proliferative responses and impaired antitumor activity that can be partially restored by antibody-mediated disruption of PD-1/programmed death ligand interaction.
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