期刊
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
卷 167, 期 8, 页码 -出版社
ELECTROCHEMICAL SOC INC
DOI: 10.1149/1945-7111/ab8922
关键词
Electrochemical aptasensor; Thrombin; MoS2 nanoparticles; Iron-porphyrinic metal-organic framework; Signal amplification
资金
- Advanced Catalysis and Green Manufacturing Collaborative Innovation Center (Changzhou University) [213164]
An ultrasensitive electrochemical aptasensor for thrombin (TB) was designed using MoS2 nanoparticles loaded iron-porphyrinic metal-organic framework (PCN-223-Fe) as the electrochemical tracer. Spindle-shaped PCN-223-Fe was synthesized with zirconium ion as node and iron-porphyrin as linker, and in situ loaded with MoS2 nanoparticles to obtain MoS2/PCN-223-Fe composite. MoS2/PCN-223-Fe was characterized by electron microscopies, X-ray diffraction and X-ray photoelectron spectroscopy. Due to the synergistic effect of MoS2 and PCN-223-Fe, MoS2/PCN-223-Fe modified electrode exhibited enhanced electrocatalytic activity to oxygen reduction. Sequentially, MoS2/PCN-223-Fe was combined with TB aptamer 2 to prepare signal probe. A sandwich-type electrochemical aptasensor was constructed for TB detection. TB aptamer 1 was fixed on electrode surface to capture TB molecules, and then TB aptamer 2 functionalized MoS2/PCN-223-Fe tracer was introduced on electrode surface. By detecting the electrochemical signal of oxygen reduction catalyzed by MoS2/PCN-223-Fe, the designed TB sensor was monitored. The proposed TB aptasensor showed a wide linear range from 0.1 pM to 100 nM and a low detection limit of 0.03 pM (S/N = 3). Meanwhile, the aptasensor showed good reproducibility, stability and selectivity, which was successfully applied for TB detection in human serum sample. This strategy broadens application of metal-organic frameworks, and could be used for other biomolecules detection. (C) 2020 The Electrochemical Society (ECS). Published on behalf of ECS by IOP Publishing Limited.
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