期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 23, 页码 4634-4641出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b01567
关键词
piceatannol; TNF-alpha; 3T3-L1 adipocytes; inflammation; insulin resistant
资金
- National High Technology Research and Development Program of China [2013AA102202, 2013AA102207]
- Foundation for Young Scientist of Beijing Technology & Business University [QNJJ2017-07]
- Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU)
- Beijing Excellent Talents Funding for the Youth Scientist Innovation Team
Piceatannol, a bioactive component in grape and blueberry, was examined for its potential in decreasing the inflammatory activities in adipocytes using a cocultured adipocyte and macrophage system, and suppressing tumor necrosis factor-alpha (TNF-alpha)-mediated inflammation and the related insulin resistance using a 3T3-L1 adipocyte model. Piceatannol at 10 mu M significantly reduced the release of inflammatory cytokines of TNF-alpha and monocyte chemoattractant protein-1 (MCP-1) by 19 and 31% in the cocultured system, respectively. Pretreatment with piceatannol also inhibited TNF-alpha-induced expression of interleukin-6 (IL-6) and MCP-1 at both mRNA and protein levels in the 3T3-L1 adipocytes. Piceatannol also partially improved the malfunction of insulin-stimulated glucose uptake, which was reduced by TNF-alpha in 3T3-L1 adipocytes. Furthermore, the inhibitions were mediated by significant blocking of I kappa B alpha phosphorylation and nuclear factor-kappa B (NF-kappa B) activation through suppressing nuclear translocation of NF-kappa B p65 along with c-Jun N-terminal kinase (JNK)-mitogen activated protein kinase (MAPK) activation. In addition, the Akt-dependent forkhead box O1 (FoxO1) signaling pathway was involved in the restoration of insulin-stimulated glucose uptake through suppressing the down-regulation of phosphorylation of Akt and FoxO1 expressions. These results suggested the potential of piceatannol in improving chronic inflammatory condition and insulin sensitivity in obese adipose tissues.
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