期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 31, 页码 6638-6646出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b02507
关键词
naringenin; Saccharomyces cerevisiae; metabolic engineering; tyrosine
资金
- Nanyang Technological University Singapore (iFood Research grant)
Flavonoids are an important class of plant polyphenols that possess a variety of health benefits. In this work, S. cerevisiae was metabolically engineered to produce the flavonoid naringenin, using tyrosine as the precursor. Our strategy to improve naringenin production comprised three modules. In module 1, we employed a modified GAL system to overexpress the genes of the naringenin biosynthesis pathway and investigated their synergistic action. In module 2, we simultaneously up-regulated acetyl-CoA production and down-regulated fatty acid biosynthesis in order to increase the precursor supply, malonyl-CoA. In module 3, we engineered the tyrosine biosynthetic pathway to eliminate the feedback inhibition of tyrosine and also down-regulated competing pathways. It was found that modules 1 and 3 played important roles in improving naringenin production. We succeeded in producing up to similar to 90 mg/L of naringenin in our final strain, which is a 20-fold increase as compared to the parental strain.
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