期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 12, 页码 2495-2502出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b05308
关键词
selenomethionine; AFB1; cytochrome P450 enzymes; siRNA; selenoprotein W; primary chicken hepatocytes
资金
- National Natural Science Foundation of China (NFSC) [31472253, 31472252]
- Fundamental Research Funds for the Central Universities [Y0201500198]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (Jiangsu, China)
- Biotechnology and Biological Sciences Research Council [1368759] Funding Source: researchfish
This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell-viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >.05 mu g/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however; did not affect CYP450 3A37 mRNA levels. Supplementation with 2 mu M SeMet protected against AFB1 induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in priinary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
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