期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 48, 页码 10638-10650出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b04639
关键词
processed animal proteins (PAPs); bovine spongiform encephalopathy (BSE); signature peptides; LC-QqLIT MS; bovine; pig; fish
资金
- Italian Ministry of Health. Project Convenzione tra il Ministero della Salute e l'Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta per la sorveglianza sui costituenti di origine animale nei mangimi [16 CEA-CUP J18C16000520001]
An innovative analytical strategy has been applied to identify signature peptides able to distinguish among processed animal proteins (PAPs) derived from bovine, pig, fish, and milk products. Proteomics was first used to elucidate the proteome of each source. Starting from the identified proteins and using a funnel based approach, a set of abundant and well characterized peptides with suitable physical-chemical properties (signature peptides) and specific for each source was selected. An on-target LC-ESI-MS/MS method (MRM mode) was set up using standard peptides and was then applied to selectively identify the PAP source and also to distinguish proteins from bovine carcass and milk proteins. We believe that the method described meets the request of the European Commission which has developed a strategy for gradually lifting the total ban toward species to species ban, therefore requiring official methods for species-specific discrimination in feed.
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