Impact of Mechanical and Thermal Energies on the Degradation of T-2 and HT-2 Toxins during Extrusion Cooking of Oat Flour

The type A trichothecenes T-2 toxin (T-2) and HT-2 toxin (HT-2) are naturally occurring toxic food contaminants, with the highest concentrations found in contaminated oats. The influence of thermal food processing on these toxins is poorly understood, and only a few publications address the degradation rates. Therefore, we systematically investigated the degradation of T-2 and HT-2 during both laboratory and industrial-scale extrusion cooking of oats. Extrusion cooking under. laboratory conditions was performed with oats fortified with T-2 or HT-2 as well as with naturally contaminated oat flour dust. The experiments were designed according to industrial conditions in terms of temperature, water content, pressure, residence time, and oat content. Flour mixtures containing naturally contaminated oats were used for industrial-scale processing. Degradation rates under laboratory conditions were up to 59.6 +/- 1.51 and 47.2 +/- 0.53% for T-2 and HT-2, respectively, in fortified extrudates but were decreased to 35.1 +/- 1.55 and 22.0 +/- 4.68% when naturally contaminated flour samples were used. The results show a higher degradation of T-2 during extrusion cooking than of HT-2. Moisture content, mechanical shear, and temperature showed an impact on the toxin degradation and can be optimized to counteract food contamination.