期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 51, 页码 11202-11211出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b04442
关键词
silkworm pupa protein; silkworm excrement; moderately hydrophilic peptide; ACE-inhibitory activity; ultrafast screening; molecular docking
资金
- National Natural Science Foundation of China [31401629, 21666004, 21676059, 21606054]
- Guangxi Distinguished Experts Special Foundation of China
- Natural Science Foundation of Guangxi Zhuang Autonomous Region, China [2016GXNSFAA380229, 2017GXNSFFA198009]
- Scientific Research Foundation of Guangxi University [XGZ130963]
- Innovation and Entrepreneurship Training Program of Guangxi Zhuang Autonomous Region [201710593185]
A novel, moderately hydrophilic peptide (RYL) with high ACE-inhibitory activity was screened ultrafast via a concept of waste conversion using waste. This novel peptide was screened from silkworm pupa using an Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement. FL/Z-SE possessed magnetic properties and specific selection for peptides due to Fe's dual functions. The selected RYL, which has moderate hydrophilicity (LogP = -0.22), exhibited a comparatively high ACE-inhibitory activity (IC50 = 3.31 +/- 0.11 mu M). The inhibitory kinetics and docking-simulation results show that, as a competitive ACE inhibitor, RYL formed five hydrogen bonds with the ACE residues in the S1 and S2 pockets. In this work, both the screening carbon material and the selected ACE-inhibitory peptide were derived from agricultural waste (silkworm excrement and pupa), which offers a new way of thinking about the development of advanced uses of the silkworm byproducts and wastes.
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