期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 8, 页码 1630-1640出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b05037
关键词
cold-active cellulase; Cryptopygus antarcticus; endo-beta-1,4-glucanase; horizontal gene transfer; biochemical and structural features; biofuel production from seaweeds
资金
- KIOST in-house programs [PE99413, PE98933, PO00110]
- National Research Foundation of Korea Grants [NRF-2015R1A2A2A01004168, NRF-2015M1A5A1037480]
- Marine Biotechnology Program - Ministry of Oceans and Fisheries, Korea [PJT200620]
The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-,beta-1,4-glucanases. The organization of five introns within the open reading frame of the CaCel gene indicates its endogenous origin in the genome of the species, which suggests the horizontal transfer of the gene from fungi to the springtail. CaCel exhibited optimal activity at pH 3.5, retained 80% of its activity at 0-10 degrees C, and maintained a half-life of 4 h at 70 degrees C. Based on the structural comparison between CaCel and a fungal homologue, we deduced the structural basis for the unusual characteristics of CaCel. Under acidic conditions at 50 degrees C, CaCel was effective to digest the green algae (Viva pertusa), suggesting that it could be exploited for biofuel production from seaweeds.
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