期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 41, 页码 9078-9086出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b03148
关键词
edible fungi; elicitation; deglycosylation; hairy root cultures; isoflavone aglycones; antioxidant activity
资金
- National Key R&D Program of China [2017YFD0600205]
- Fundamental Research Funds for the Central Universities [2572017DA04]
- Heilongjiang Province Science Foundation for Youths [QC2017012]
- Northeast Forestry University [1020160010]
- Double First-Rate Special Funds [41112432]
A cocultivation system of Astragalus membranaceus hairy root cultures (AMHRCs) and immobilized food-grade fungi was established for the enhanced production of calycosin (CA) and formononetin (FO). The highest accumulations of CA (730.88 +/- 63.72 mu g/g DW) and FO (1119.42 +/- 95.85 mu g/g DW) were achieved in 34 day-old AMHRCs cocultured with immobilized A. niger (IAN) for 54 h, which were 7.72- and 18.78-fold higher than CA and FO in nontreated control, respectively. IAN deglycosylation could promote the formation of CA and FO by conversion of their glycoside precursors. IAN elicitation could intensify the generation of endogenous signal molecules involved in plant defense response, which contributed to the significantly up-regulated expression of genes in CA and FO biosynthetic pathway. Overall, the coupled culture of IAN and AMHRCs offered a promising and effective in vitro approach to enhance the production of two health-promoting isoflavone aglycones for possible nutraceutical and pharmaceutical uses.
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