期刊
CYTOGENETIC AND GENOME RESEARCH
卷 145, 期 1, 页码 50-58出版社
KARGER
DOI: 10.1159/000381321
关键词
Bisphenol A; Bovine; Chromosome alignment; Meiotic abnormalities; Oocyte maturation; Oocyte quality; Spindle morphology
资金
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
- Ontario Veterinary College scholarship
- Ontario Graduate Scholarship
Bisphenol A (BPA) exposure in humans is widespread, and BPA has been detected in a variety of samples including follicular fluid. BPA levels have been found to negatively correlate with the developmental potential of oocytes in women undergoing in vitro fertilization and to induce meiotic abnormalities experimentally in human and mouse models. BPA may detrimentally affect oocyte maturation, and different concentrations of exposure can cause various outcomes. Because of the importance of oocyte maturation on developmental potential, disturbances during this time can significantly impact oocyte viability. Here, bovine oocytes were matured in vitro with and without BPA treatment of the media. The levels of BPA taken up by the oocytes were much lower than the initial exposure. Medium treatment with 30 ng/ml resulted in an average of 2.48 ng/ml BPA measured in mature oocytes. These oocytes exhibited decreased maturation and increased incidence of spindle abnormalities. Only 57.4% of oocytes exposed to 30 ng/ml BPA reached maturity compared to 72.4% of controls (p < 0.05). Mature oocytes following BPA exposure displayed increased abnormal spindle morphology (67.9%) and chromosome dispersal (60%) compared to all other groups analyzed (p < 0.05). Thus, exposure to BPA during in vitro oocyte maturation has the potential to decrease oocyte quality. (C) 2015 S. Karger AG, Basel
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