期刊
CHEMICAL SCIENCE
卷 11, 期 17, 页码 4410-4415出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0sc01009j
关键词
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资金
- Cancer Prevention and Research Institute of Texas (CPRIT) [RR170014]
- Robert A. Welch Foundation [C-1970]
- Hamill Innovation Award (Hamill Foundation)
- John S. Dunn Foundation Collaborative Research Award (Gulf Coast Consortia)
- NIH [R35-GM133706]
Light-activated fluorescence affords a powerful tool for monitoring subcellular structures and dynamics with enhanced temporal and spatial control of the fluorescence signal. Here, we demonstrate a general and straightforward strategy for using a tetrazine phototrigger to design photoactivatable fluorophores that emit across the visible spectrum. Tetrazine is known to efficiently quench the fluorescence of various fluorophores via a mechanism referred to as through-bond energy transfer. Upon light irradiation, restricted tetrazine moieties undergo a photolysis reaction that generates two nitriles and molecular nitrogen, thus restoring the fluorescence of fluorophores. Significantly, we find that this strategy can be successfully translated and generalized to a wide range of fluorophore scaffolds. Based on these results, we have used this mechanism to design photoactivatable fluorophores targeting cellular organelles and proteins. Compared to widely used phototriggers (e.g., o-nitrobenzyl and nitrophenethyl groups), this study affords a new photoactivation mechanism, in which the quencher is photodecomposed to restore the fluorescence upon light irradiation. Because of the exclusive use of tetrazine as a photoquencher in the design of fluorogenic probes, we anticipate that our current study will significantly facilitate the development of novel photoactivatable fluorophores.
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