期刊
PHARMACEUTICAL BIOLOGY
卷 58, 期 1, 页码 400-409出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2020.1759654
关键词
Human dermal papilla cells; anti-apoptotic; beta-catenin; GSK3 beta; Bcl-2; Bax; ERK; Akt; C57BL/6 mice; hair regrowth
资金
- Basic Science Research Programme through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [NRF-2017R1D1A1B03033507]
- Chung-Ang University
Context: Although Salvia plebeia (SP) R. Brown (Labiatae) is known to possess various biological activities, the effects of SP on hair growth have not been elucidated. Objective: To investigate the hair growth potential of SP extract by using human dermal papilla cells (hDPCs) and C57BL/6 mice. Materials and methods: The entire SP plant sample was ground into powder and extracted with 99.9% methyl alcohol. Various concentrations of SP extract were added to hDPCs to evaluate the proliferation, migration, and factors related to hair growth and cycling. Effect of topical SP administration on hair regrowth was tested in vivo in male C57BL/6 mice for 21 days. Results: SP extract significantly increased the proliferation of cultured hDPCs at doses of 15.6 and 31.3 mu g/mL compared to control group by 123% and 132%, respectively. Expression of hepatocyte growth factor increased while the level of TGF-beta 1 and SMAD2/3 decreased when treated with SP extract. At the molecular level, the extract activated Wnt/beta-catenin signalling by raising beta-catenin and phospho-GSK3 beta expression. SP extract also exerted anti-apoptotic and proliferative effects in hDPCs by increasing the Bcl-2/Bax ratio and activating cell proliferation-related proteins, ERK and Akt. Finally, the extract caused an induction of the anagen phase leading to significantly enhanced hair growth in treated male mice. Discussion and conclusion: Our results indicate that SP extract has the capacity to activate hDPCs into a proliferative state to promote hair growth. Further research is necessary to determine the bioactive components and their mechanisms of action responsible for SP-related hair growth effect.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据