期刊
BIOMEDICINES
卷 8, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines8040081
关键词
multi-omics; cervical epithelial microenvironment; HPV; 16S rRNA; metabolomics
资金
- Puerto Rico Science Technology Research Trust
- Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health [P20 GM103475]
- Advanced Clinical and Translational Research Award of the Hispanics-in-Research Capability (HiREC) Endowment of the National Institute On Minority Health and Health Disparities of the National Institutes of Health (NIMHD) [S21MD001830]
- NIMHD Center for Collaborative Research in Minority Health and Health Disparities (CCRMHD) [2U54MD007600]
The multi-omic integration of microbiota data with metabolomics has gained popularity. This protocol is based on a human multi-omics study, integrating cervicovaginal microbiota, HPV status and neoplasia, with urinary metabolites. Indeed, to understand the biology of the infections and to develop adequate interventions for cervical cancer prevention, studies are needed to characterize in detail the cervical microbiota and understand the systemic metabolome. This article is a detailed protocol for the multi-omic integration of cervical microbiota and urine metabolome to shed light on the systemic effects of cervical dysbioses associated with Human Papillomavirus (HPV) infections. This methods article suggests detailed sample collection and laboratory processes of metabolomics, DNA extraction for microbiota, HPV typing, and the bioinformatic analyses of the data, both to characterize the metabolome, the microbiota, and joint multi-omic analyses, useful for the development of new point-of-care diagnostic tests based on these approaches.
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