期刊
LIFE-BASEL
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/life10020017
关键词
directed evolution; enrichment factor; self-selection
资金
- ERC CoG Grant ProFF [647275]
- European Research Council (ERC) [647275] Funding Source: European Research Council (ERC)
High-throughput, in vitro approaches for the evolution of enzymes rely on a random micro-encapsulation to link phenotypes to genotypes, followed by screening or selection steps. In order to optimise these approaches, or compare one to another, one needs a measure of their performance at extracting the best variants of a library. Here, we introduce a new metric, the Selection Quality Index (SQI), which can be computed from a simple mock experiment, performed with a known initial fraction of active variants. In contrast to previous approaches, our index integrates the effect of random co-encapsulation, and comes with a straightforward experimental interpretation. We further show how this new metric can be used to extract general protocol efficiency trends or reveal hidden selection mechanisms such as a counterintuitive form of beneficial poisoning in the compartmentalized self-replication protocol.
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