Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Blend Obtained through the Co-culture of Cupriavidus Necator and Pseudomonas Citronellolis in Apple Pulp Waste

The co-culture ofCupriavidus necatorDSM 428 andPseudomonas citronellolisNRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture's biomass, resulting in a natural blend that was composed of around 48 wt% P(3HB) and 52 wt% mcl-PHA, with an average molecular weight of 4.3 x 10(5)Da and a polydispersity index of 2.2. Two melting temperatures (T-m) were observed for the blend, 52 and 174 degrees C, which correspond to the T(m)of the mcl-PHA and P(3HB), respectively. P(3HB)/mcl-PHA blend films prepared by the solvent evaporation method had permeabilities to oxygen and carbon dioxide of 2.6 and 32 Barrer, respectively. The films were flexible and easily deformed, as demonstrated by their tensile strength at break of 1.47 +/- 0.07 MPa, with a deformation of 338 +/- 19% until breaking, associated with a Young modulus of 5.42 +/- 1.02 MPa. This study demonstrates for the first time the feasibility of using the co-culture ofC. necatorandP. citronellolisstrains to obtain a natural blend of P(3HB)/mcl-PHA that can be processed into films suitable for applications ranging from commodity packaging products to high-value biomaterials.