期刊
ISME JOURNAL
卷 12, 期 3, 页码 838-848出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41396-017-0010-z
关键词
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资金
- Japan Society for the Promotion of Science [LR033]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [26103527]
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) KAKENHI [15H05638]
- JSPS Fellowship for Japan Junior Scientists [15J12274]
- Postdoctral Fellowship for Research Abroad
- Grants-in-Aid for Scientific Research [15H05638, 15H04364] Funding Source: KAKEN
A bean bug symbiont, Burkholderia sp. RPE64, selectively colonizes the gut crypts by flagella-mediated motility: however, the mechanism for this colonization remains unclear. Here, to obtain clues to this mechanism, we characterized the swimming motility of the Burkholderia symbiont under an advanced optical microscope. High-speed imaging of cells enabled the detection of turn events with up to 5-ms temporal resolution, indicating that cells showed reversal motions (theta similar to 180 degrees) with rapid changes in speed by a factor of 3.6. Remarkably, staining of the flagellar filaments with a fluorescent dye Cy3 revealed that the flagellar filaments wrap around the cell body with a motion like that of a ribbon streamer in rhythmic gymnastics. A motility assay with total internal reflection fluorescence microscopy revealed that the left-handed flagellum wound around the cell body and propelled it forward by its clockwise rotation. We also detected periodic-fluorescent signals of flagella on the glass surface, suggesting that flagella possibly contacted the solid surface directly and produced a gliding-like motion driven by flagellar rotation. Finally, the wrapping motion was also observed in a symbiotic bacterium of the bobtail squid, Aliivibrio fischeri, suggesting that this motility mode may contribute to migration on the mucus-filled narrow passage connecting to the symbiotic organ.
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